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Introduction to Chromatography (10 mins):
- Overview of chromatography: definition and historical context.
- Importance in analytical chemistry and various industries, such as pharmaceuticals, environmental science, and food safety.
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High-Performance Liquid Chromatography (HPLC) (10 mins):
- Basic principles and mechanisms of HPLC.
- Key applications, including drug development, quality control, and toxin analysis.
- Detailed overview of HPLC instrumentation, including columns, detectors, and solvents.
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Gas Chromatography (GC) (10 mins):
- Fundamentals and operational principles of GC.
- Typical applications, such as petrochemical analysis, forensic science, and environmental monitoring.
- Description of GC equipment, including injectors, columns, and detectors.
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Liquid Chromatography-Mass Spectrometry (LCMS) (10 mins):
- Combining liquid chromatography with mass spectrometry for enhanced analytical capabilities.
- Benefits such as high sensitivity, specificity, and quantitative analysis.
- Detailed overview of LCMS instrumentation and example applications in metabolomics and proteomics.
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Gas Chromatography-Mass Spectrometry (GCMS) (10 mins):
- Integrating gas chromatography with mass spectrometry for detailed compound identification.
- Advantages and real-world applications, including pesticide analysis and drug testing.
- Description of GCMS systems, including ion sources and mass analyzers.
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Q&A and Discussion (10 mins):
- Addressing students' questions and clarifying concepts.
- Discussion on the relevance, advancements, and future trends in chromatography and mass spectrometry.